Description
This volume covers methods for determination of autoantibodies in rheumatic connective tissue diseases and organ-specific diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Autoantibodies: Methods and Protocols aims to be helpful for all persons working with research and development of autoimmune laboratory diagnostics and for clinicians using autoantibody tests in daily work with patients. 1. Autoantibodies as Diagnostic Tools Gunnar Houen 2. Non-specific Binding in Immunoassays for Autoantibodies Gunnar Houen 3. Detection of Autoantibodies by Indirect Immunofluorescence Cytochemistry on HEp-2 Cells Alessandra Dellavance and Luis Eduardo Coelho Andrade 4. Detection of Anti-Neutrophil Cytoplasmic Antibodies by Indirect Immunofluorescence J. J. B. C. van Beers, J. Vanderlocht, C. Roozendaal, and Jan G. M. C. Damoiseaux 5. Determination of Subset-Restricted Anti-Neutrophil Cytoplasmic Antibodies (ANCA) by Immunofluorescence Cytochemistry Firoozeh Amirbeagi, Amanda Welin, Pontus Thulin, and Johan Bylund 6. Determination of Anti-aquaporin 5 Autoantibodies by Immunofluorescence Cytochemistry Jehan Alam, Sumin Jeon, and Youngnim Choi 7. Determination of cN1A Autoantibodies by Cell-Based Immunofluorescence Cytochemistry Satoshita Yamashita and Nozomu Tawara 8. Determination of Agonistically Acting Autoantibodies to the Adrenergic Beta-1 Receptor by Cellular Bioassay Hanna Davideit, Annekathrin Haberland, Sabine Bartel, Sarah Schulze-Rothe, Johannes Mller,and Katrin Wenzel 9. Determination of Autoantibodies to Salivary Gland Antigens Li Cui, Xinyuan Zhao, and Shen Hu 10 Measurement of autoantibodies to the gastric H+,K+-ATPase (ATP4A/B) using a Luciferase Immuno Precipitation System (LIPS) Edith Lahner, Ilaria Marzinotto, Cristina Brigatti, Howard Davidson, Janet Wenzlau, Lorenzo Piemonti, Bruno Annibale, and Vito Lampasona 11. Detection of DNA Autoantibodies by Electrophoretic Mobility Shift Assay Jacqueline Keyhani and Ezzatollah Keyhani 12. Determination of Autoantibodies to Neutrophil Granule Constituents by Solid Phase Immunoassay J. Vanderlocht, J.J.B.C. van Beers, P.C. Limburg, Jan G. M. C. Damoiseaux, and C. Roozendaal 13. Determination of Autoantibodies to C3 Nephritic Factors by ELISA Lillemor Skattum 14. Determination of Anti-C1q Autoantibodies by Western Blot Anci Verlemyr, Lennart truedsson, and Lillemor Skattum 15. Anti-Factor H Autoantibodies Assay by ELISA Marie Snant and Marie-Agnes Durey 16. Determination of Autoantibodies to Transglutaminase by Electrochemiluminescence (ECL) Assay Zhiyuan Zhao, Yong Gu, Dongmei Miao, Eric Hoffmeyer, Yu Liu, and Liping Yu 17. Determination of CRP Autoantibodies by SPR Immunoassay Li Qiu-yu and Li Hai-yun 18. Histone Protein Epitope Mapping for Autoantibody Recognition in Rheumatoid Arthritis Felicia Real-Fernandez, Federico Pratesi, Paola Migliorini, and Paolo Rovero 19. Detection of SSA and SSB Autoantibodies by Enzyme-Linked Immunosorbent Assay Nicole Hartwig Trier 20. Assessment of Peptidylarginine Deiminase Activity by ELISA using Human Fibrinogen as Substrate Dres Damgaard and Claus H. Nielsen 21. The Use of a Citrullinated Peptide Panel for Detection of Anti-Citrullinated Protein Antibodies by Enzyme-Linked Immunosorbent Assay Nicole H. Trier 22. Determination of Autoantibodies by Line Blotting Louise Sternbk, Tina Friis, and Gunnar Houen 23. Determination of Rheumatoid Factors by ELISA Nicole H. Trier and Gunnar Houen 24. Detection of autoantibodies to complement components by surface plasmon resonance-based technology Remi Noe, Sophie Chauvet, Shambhuprasad K. Togarsimalemath, Maria Chiara Marinozzi, Maria Radanova, Vasil V. Vasilev, Veronique Fremeaux-Bacchi, Marie-Agnes Dragon-Durey, and Lubka T. Roumenina
