Description
This extensive volume covers basic and advanced aspects of peptide antibody production, characterization and uses. Although peptide antibodies have been available for many years, they continue to be a field of active research and method development. For example, peptide antibodies which are dependent on specific posttranslational modifications are of great interest, such as phosphorylation, citrullination and others, while different forms of recombinant peptide antibodies are gaining interest, notably nanobodies, single chain antibodies, TCR-like antibodies, among others. Within this volume, those areas are covered, as well as several technical and scientific advances: solid phase peptide synthesis, peptide carrier conjugation and immunization, genomics, transcriptomics, proteomics and elucidation of the molecular basis of antigen presentation and recognition by dendritic cells, macrophages, B cells and T cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Peptide Antibodies: Methods and Protocols serves as an ideal reference for researchers exploring this vital and expansive area of study. 1. Peptide Antibodies: Past, Present, and Future Gunnar Houen 2. The Structure of Natural and Recombinant Antibodies Hui Ma and Richard O’Kennedy 3. Prediction of Antigenic B and T Cell Epitopes Via Energy Decomposition Analysis: Description of the Web-Based Prediction Tool BEPPE Claudio Peri, Oscar C. Sol, Dario Corrada, Alessandro Gori, Xavier Daura, and Giorgio Colombo 4. Prediction of Antibody Epitopes Morten Nielsen and Paolo Marcatili 5. Fmoc Solid-Phase Peptide Synthesis Paul Robert Hansen and Alberto Oddo 6. Peptide-Carrier Conjugation Paul Robert Hansen 7. Solid Phase Peptide-Carrier Conjugation Gunnar Houen and Dorthe T. Olsen 8. Analysis of Peptides and Conjugates by Amino Acid Analysis Peter Hjrup 9. Characterization of Synthetic Peptides by Mass Spectrometry Bala K. Prabhala, Osman Mirza, Peter Hjrup, and Paul Robert Hansen 10. Interpretation of Tandem Mass Spectrometry (MSMS) Spectra for Peptide Analysis Karin Hjern and Peter Hjrup 11. Polyclonal Peptide Antisera Tina H. Pihl, Kristin E. Engelhart, and Gunnar Houen 12. Production and Screening of Monoclonal Peptide Antibodies Nicole Hartwig Trier, Anne Mortensen, Annette Schiolborg, and Tina Friis 13. Production of Epitope-Specific Antibodies by Immunization with Synthetic Epitope Peptide Formulated with CpG-DNA-Liposome Complex Without Carriers Dongbum Kim, Younghee Lee, and Hyung-Joo Kwon 14. Thioredoxin-Displayed Multipeptide Immunogens Angelo Bolchi, Elena Canali, Andrea Santoni, Gloria Spagnoli, Daniele Viarisio, Rosita Accardi, Massimo Tommasino, Martin Mller, and Simone Ottonello 15. The Purification of Natural and Recombinant Peptide Antibodies by Affinity Chromatographic Strategies Hui Ma and Richard O’Kennedy 16. Isolation of Camelid Single-Domain Antibodies Against Native Proteins Using Recombinant Multivalent Peptide Ligands Norah A. Alturki, Kevin A. Henry, C. Roger MacKenzie, and Mehdi Arbabi-Ghahroudi 17. Generation of TCR-Like Antibodies Using Phage Display Brian H. Santich, Hong Liu, Cheng Liu, and Nai-Kong V. Cheung 18. Structural Characterization of Peptide Antibodies Anna Chailyan and Paolo Marcatili 19. Automated High Throughput Mapping of Linear B Cell Epitopes Using a Statistical Analysis of High Density Peptide Microarray Data Thomas sterbye and Sren Buus 20. Characterization of Peptide Antibodies by Epitope Mapping Using Resin-Bound and Soluble Peptides Nicole Hartwig Trier 21. Screening and Characterization of Linear B-Cell Epitopes by Biotinylated Peptide Libraries Ida Rosenkrands and Anja Olsen 22. Bead-Based Peptide Arrays for Profiling the Specificity of Modification State-Specific Antibodies Angela Filomena, Yvonne Heubach, Markus F. Templin, Thomas O. Joos, Nicole Schneiderhan-Marra, and Oliver Poetz 23. Surface Plasmon Resonance Method to Evaluate Anti-Citrullinated Protein/Peptide Antibody Affinity to Citrullinated Peptides Feliciana Real-Fernndez, Giada Rossi, Filomena Panza, Federico Pratesi, Paola Migliorini, and Paolo Rovero 24. Specificity Analysis of Histone Modification Specific Antibodies or Reading Domains on Histone Peptide Arrays Goran Kungulovski, Ina Kycia, Rebekka Mauser, and Albert Jeltsch 25. Prion-Specific Antibodies Produced in Wild-Type Mice Peter M.H. Heegaard, Ann-Louise Bergstrm, Heidi Gertz Andersen, and Henriette Cordes 26. Immunoblotting with Peptide Antibodies: Differential Immunoreactivities Caused by Certain Amino Acid Substitutions in a Short Peptide, and Possible Effects of Differential Refolding of the Peptide on a Nitrocellulose or PVDF Membrane Takenori Yamamoto, Taisuke Matsuo, Atsushi Yamamoto, Ryohei Yamagoshi, Kazuto Ohkura, Masatoshi Kataoka, and Yasuo Shinohara 27. Immunocytochemical and Immunohistochemical Staining with Peptide Antibodies Tina Friis, Klaus Boberg Pedersen, David Hougaard, and Gunnar Houen 28. Designing B-Cell Epitopes for Immunotherapy and Subunit Vaccines Harinder Singh, Sudheer Gupta, Ankur Gautam, and Gajendra P.S. Raghava 29. Enterovirus-Specific Anti-Peptide Antibodies Chit Laa Poh, Katherine Kirk, Hui Na Chua, and Lara Grollo 30. Therapeutic HIV Peptide Vaccine Anders Fomsgaard
