Description
Preface… Table of Contents… Contributing Authors… 1. An Overview on Selectin Marker Genes for Transformation of Saccharomyces cerevisiae Verena Siewers 2. High Efficiency Plasmid DNA Transformation in Yeast O’Taveon R. Fitzgerald, Nestor D. Rodriguez, and L. Kevin Lewis 3. CasPER, A CRISPR/Cas9-Based Method for Directed Evolution in Genomic loci in Saccharomyces cerevisiae Tadas Jakociunas, Michael K. Jensen, and Jay D. Keasling 4. Fluorescence Activated Cell Sorting as a Tool for Recombinant Strain Screening Christos Skrekas, Raphael Ferreira, and Florian David 5. Generation of Arming Yeasts with Active Proteins and Peptides via Cell Surface Display System: Cell Surface Engineering, Bio-Arming Technology Kouichi Kuroda and Mitsuyoshi Ueda 6. Strains and Molecular Tools for Recombinant Protein Production in Pichia pastoris Claudia Rinnofner, Michael Felber, and Harald Pichler 7. Competent Cell Preparation and Transformation of Pichia pastoris Joan Lin-Cereghino, Christopher A. Naranjo, and Geoff P. Lin-Cereghino 8. CRISPR/Cas9 Tool Kit for Efficient and Targeted Insertion/Deletion Mutagenesis of the Komagataella phaffii (Pichia pastoris) Genome Jasmin Elgin Fischer and Anton Glieder 9. Plasmid-Based Gene Knockout Strategy with Subsequent Marker Recycling in Pichia pastoris Simon Kobalter, Astrid Radkohl, Helmut Schwab, Anita Emmerstorfer-Augustin, and Harald Pichler 10. Engineering of Promoters for Gene Expression in Pichia pastoris Thomas Vogl 11. Molecular Tools for Leveraging the Potential of the Acid Tolerant Yeast Zygosaccharomyces bailii as Cell Factory Paola Branduardi, Liliane Barroso, Laura Dato, Edward J. Louis, and Danilo Porro 12. Golden Gate Multi-Genes Assembly Method for Yarrowia lipolytica Macarena Larroude, Jean-Marc Nicaud, and Tristan Rossignol 13. A Universal Gene Expression System for Novel Yeast Species Dominik Majzita, Anssi Rantasalo, and Marja Ilmn 14. Dynamic Feeding of Pichia pastoris Alexander Pekarsky and Oliver Spadiut 15. Automated Evolutionary Engineering of Yeasts Erik de Hulster, Christiaan Mooiman, Rowin Timmermans, and Robert Mans 16. Reconstruction of Genome-Scale Metabolic Model of Hansenula polymorpha using RAVEN Francisco Zorrilla and Eduard J. Kerkhoven Subject Index List…
