Description
Myc controls multiple cellular functions, including cell proliferation, growth, differentiation and death, both directly and indirectly, through its modulation of downstream transcriptional programs. In The Myc Gene: Methods and Protocols, experts in the field summarize the standard and novel techniques that allow the studying of Myc mechanism of action in normal and cancer cells, in vitro and in vivo, in one succinct manual. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. 1. The Myc World Within Reach Nicole M. Sodir and Laura Soucek 2. Methods for the Expression, Purification, Preparation and Biophysical Characterization of Constructs of the c-Myc and Max b-HLH-LZs Marie-Eve Beaulieu, Franois-Olivier Mc Duff, Mikal Bdard, Martin Montagne and Pierre Lavigne 3. Analyzing Myc in Cell Transformation and Evolution Markus Hartl and Klaus Bister 4. Identifying Myc Interactors Romina Ponzielli, William B. Tu, Igor Jurisica, and Linda Z. Penn 5. Detection of c-Myc Protein-Protein Interactions And Phosphorylation Status by Immunoprecipitation Colin J. Daniel, Xiaoli Zhang and Rosalie C. Sears 6. An Efficient Way of Studying Protein-Protein Interactions Involving HIF-a, c-Myc, and Sp1 Kenneth K.-W. To and L. Eric Huang 7. Methods for Determining Myc-induced Apoptosis Dan Lu and Trevor D. Littlewood 8. Methods to Study Myc-regulated Cellular Senescence Vedrana Tabor, Matteo Bocci and Lars-Gunnar Larsson 9. Chromatin Immunoprecipitation Assays for Myc and N-Myc Bonnie L. Barrilleaux, Rebecca Cotterman, and Paul S. Knoepfler 10. Methods to Quantify microRNAs in the Myc Gene Network for Posttranscriptional Gene Repression Rui Song, Nicole Sponer, and Lin He 11. Genome-wide Analysis of c-MYC-regulated mRNAs and miRNAs, and c-MYC DNA-binding by Next Generation Sequencing Rene Jackstadt, Antje Menssen and Heiko Hermeking 12. A High Throughput siRNA Screening Platform to Identify MYC Synthetic Lethal Genes as Candidate Therapeutic Targets Carla Grandori 13. Investigating Myc-dependent Translational Regulation in Normal and Cancer Cells John T. Cunningham, Michael Pourdehnad, Craig R. Stumpf, and Davide Ruggero 14. Studying Myc’s Role in Metabolism Regulation Anne Le and Chi V. Dang 15. Generation of a Tetracycline Regulated Mouse Model of MYC-induced T-cell Acute Lymphoblastic Leukemia Kavya Rakhra and Dean W. Felsher 16. Methods to Assess Myc Function in Intestinal Homeostasis, Regeneration and Tumourigenesis David J Huels, Patrizia Cammareri, Rachel A Ridgway, Jan P Medema and Owen J Sansom 17. Detection of c-myc Amplification in Formalin-fixed Paraffin-Embedded Tumor Tissue by Chromogenic in situ Hybridization (CIHS) Natasa Todorovic-Rakovic 18. Cell-Based Methods for the Identification of MYC-Inhibitory Small Molecules Catherine A. Burkhart, Michelle Haber, Murray D. Norris, Andrei V. Gudkov, and Mikhail A. Nikiforov
