Description
This volume provides readers with a wide collection of the latest and readily reproducible technical protocols available in the field of non-viral gene delivery vectors. The chapters in this book are organized into three major parts: Part I is a section on conventional bolus gene delivery vectors that introduces typical transfection approaches relying on the addition of transfectants to the cell culture medium where the cells are grown in; Part II covers stimuli-responsive bolus transfectants and topics on gene delivery complexes made of smart polymers or stimuli-responsive polymers that change according to the environment they are in and delivered by dripping into cells; Part III discusses examples of substrate-mediated gene delivery-also termed reverse transfection-and the immobilization of a gene delivery vector onto a surface as opposed to more typical bolus delivery from the medium. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Non-Viral Gene Delivery Vectors: Methods and Protocols is written for experimentalists, and is an essential part of many laboratory bookshelves. This book will help novice and professionals alike succeed in their research in this field. Part I: Conventional Bolus Gene Delivery Vectors1. Physical Chemical and Biomolecular Methods for the Optimization of Cationic Lipid-Based Lipoplexes In Vitro for the Gene Therapy Applications Santosh K. Misra and Santanu Bhattacharya2. Cationic Lipid-Based Nucleic Acid VectorsEmile Jubeli, William P. D. Goldring, and Michael D. Pungente3. Lipoplexes from Non-Viral Cationic Vectors: DOTAP-DOPE Liposomes and Gemini MicellesSara Falsini and Sandra Ristori4. Anionic/Zwitterionic Lipid-Based Gene Vectors of pDNAAna L. Barrn-Berdn, Emilio Aicart, and Elena Junquera5. Elaboration and Physicochemical Characterization of Niosome-Based Nioplexes for Gene Delivery PurposesEdilberto Ojeda, Mireia Agirre, Ilia Villate-Beitia, Mohamed Mashal, Gustavo Puras, Jon Zarate, and Jose L. Pedraz6. Quantitative Intracellular Localization of Cationic Lipid-Nucleic Acid Nanoparticles with Fluorescence MicroscopyRamsey N. Majzoub, Kai K. Ewert, and Cyrus R. Safinya7. Targeted Delivery of Peptide-Tagged DNA Lipoplexes to Hepatocellular Carcinoma CellsMario Ariatti8. Lipoplexes Strengthened by Anionic Polymers: Easy Preparation of Highly Effective siRNA Vectors Based on Cationic Lipids and Anionic PolymersDanielle Arruda, Anne Schlegel, Pascal Bigey, and Virginie Escriou9. Polymer Based Gene Silencing: In Vitro Delivery of SiRNAMargarida I. Simo Carlos, Andreas Schtzlein, and Ijeoma Uchegbu10. Polyallylamine Derivatives: Novel Non-Toxic Transfection AgentsMagdalena Wytrwal and Chantal Pichon11. Biodegradable Three-Layered Micelles and Injectable HydrogelsDaniel G. Abebe, Rima Kandil, Teresa Kraus, Maha Elsayed, Tomoko Fujiwara, and Olivia M. Merkel12. Cationic Lipid-Coated Polyplexes (“Lipopolyplexes”) for DNA and Small RNA DeliveryAlexander Ewe and Achim Aigner13. Preparation of Targeted Anionic Lipid-Coated Polyplexes for MicroRNA DeliveryXiaomeng Huang, Mengzi Zhang, Xinmei Wang, L. James Lee, and Robert J. LeePart II: Stimuli-Responsive Bolus Gene Delivery Vectors14. Characterization and Investigation of Redox-Sensitive Liposomes for Gene DeliveryDaniele Pezzoli, Elena Tallarita, Elena Rosini, and Gabriele Candiani15. From Artificial Amino Acids to Sequence-Defined Targeted OligoaminoamidesStephan Morys, Ernst Wagner, and Ulrich Lchelt16. Gene Delivery Method Using Photo-Responsive Poly(-Amino Ester) as VectorsNan Zheng, Yang Liu, and Jianjun Cheng17. Thermo-Responsive Polyplex Micelles with PEG Shells and PNIPAM Layer to Protect DNA Cores for Systemic Gene TherapyJunjie Li, Zengshi Zha, and Zhishen GePart III: Substrate-Mediated Gene Delivery18. Application of Polyethylenimine-Grafted Silicon Nanowire Arrays for Gene TransfectionHongwei Wang, Jingjing Pan, Hong Chen, and Lin Yuan
