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Preface… Table of Contents… Contributing Authors… 1. A Brief Introduction to Effector-Triggered Immunity Thomas A. Kufer and Maria Kaparakis-Liaskos 2. Lambda Red Recombineering in Shigella flexneri Adrian Herod, Julie Ryu, and John Rohde 3. Using Genomic Deletion Mutants to Investigate Effector-Triggered Immunity during Legionella pneumophila Infection Rachelia R. Wibawa, Pengfei Li, Kathleen McCaffrey, and Elizabeth L. Hartland 4. Methods of Bacterial Membrane Vesicle Production, Purification, Quantification, and Examination of their Immunogenic Functions Natalie J. Bitto and Maria Kaparakis-Liaskos 5. Gene Gun-Mediated Transient Gene Expression for Functional Studies in Plant Immunity Franz Leissing, Anja Reinstdler, Hannah Thieron, and Ralph Panstruga 6. Host Induced Gene Silencing using BPMV on Soybean to Study Genes in the Soybean Rust Fungus Phakopsora pachyrhizi Tobias I. Link 7. Virus-Mediated Protein Overexpression (VOX) in Monocots to Identify and Functionally Characterize Fungal Effectors Kostya Kanyuka 8. Time-Resolved Fluorescence Microscopy Screens on Host Protein Subversion during Bacterial Cell Invasion Lisa Sanchez, Yuen-Yan Chang, Nora Mellouk, and Jost Enninga 9. Assaying RIPK2 Activation by Complex Formation Heidrun Steinle, Kornelia Ellwanger, and Thomas A. Kufer 10. Identification of Novel Endogenous NOD Ligands: Quantitative Analysis of Binding Affinities of NOD1 or NOD2 with Sphingosine-1-Phosphate using Microscale Thermophoresis Gang Pei 11. A Surface Plasmon Resonance-Based Strategy to Characterize Interactions of NLR Proteins with Associated Factors Jonas Moecking and Matthias Geyer 12. Functional Assessment of Disease-Associated Pyrin Variants Daria Chirita, Yvan Jamilloux, Thomas Henry, and Flora Magnotti 13. An Assay for the Seeding of Homotypic Pyrin Domain Filament Transitions Inga V. Hochheiser and Matthias Geyer 14. Detection of Gasdermin Activation and Lytic Cell Death during Pyroptosis and Apoptosis Benjamin Demarco, Saray Ramos, and Petr Broz 15. Visualizing Effector Triggered Immunity in Response to Pore-Forming Toxins by Live-Cell Imaging Seong H. Chow and Thomas Naderer 16. Non-Apoptotic Cell Death Control of Neutrophil Extracellular Trap Formation Akshay A. D’Cruz, Maria Ericsson, and Ben A. Croker 17. Analysis of Bacteria-Triggered Inflammasome-Activation in Neutrophils by Immunoblot Rmi Plans, Karin Santoni, and Etienne Meunier 18. Quantifying Regulated Mitochondrial Fission in Macrophages Syeda Farhana Afroz, Nicholas D. Condon, Matthew J. Sweet, and Ronan Kapetanovic 19. Live Cell Imaging of T Cell Pyroptosis Andreas Linder and Veit Hornung Subject Index List…
