Description
This volume presents state of the art of methods that can be useful for both basic and translational researchers to conduct chemoprevention preclinical studies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cancer Chemoprevention: Methods and Protocols aims to ensure successful results in the further study of this vital field. 1. Controlled Delivery of Chemopreventive Agents by Polymeric Implants Farrukh Aqil and Ramesh C. Gupta 2. Use Of Buffy Coat Mirna Profiling for Breast Cancer Prediction in Health Women Sara Donzelli ,Giovanni Blandino, and Paola Muti 3. Micrornas in Cancer Chemoprevention: Method to Isolate them from Fresh Tissues Federica Ganci and Giovanni Blandino 4. Application of RNA-Seq Technology in Cancer Chemoprevention Frauke Goeman and Maurizio Fanciulli 5. Detection of Circulating Tumor DNA in the Blood of Cancer Patients: An Important Tool in Cancer Chemoprevention Peter Ulz, Martina Auer, and Ellen Heitzer 6. The Methylated DNA Immunoprecipitation (Medip) to Investigate the Epigenetic Remodeling in Cell Fate Determination and Cancer Development. Silvia Masciarelli, Teresa Bellissimo, Ilaria Iosue, and Francesco Fazi 7. LC-MS-Based Metabolomic Investigation Of ChemopreventivePhytochemical-Elicited Metabolic Events Lei Wang, Dan Yao,and Chi Chen 8.H NMR Metabolomic Footprinting Analysis for the In Vitro Screening of Potential Chemopreventive Agents Luca Casadei and Mariacristina Valerio 9. Comet Assay in Cancer Chemoprevention Raffaela Santoro, Maria Ferraiuolo, Gian Paolo Morgano, Paola Muti, and Sabrina Strano 10. Angiogenesis Assays Dhanya K. Nambiar, Praveen K. Kujur, and Rana P. Singh 11. ” Algimatrixtm Based 3D Cell Culture System as an In-Vitro Tumor Model: an Important Tool In Cancer Research” Chandraiah Godugu and Mandip Singh 12. Cancer Gastric Chemoprevention: Isolation Of Gastric Tumor Initiating Cells Federica Mori , Valeria Canu, Laura Lorenzon, Alfredo Garofalo, Giovanni Blandino, and Sabrina Strano 13. Isolation of Chemoresistance Cell Subpopulations Claudia Canino And Mario Cioce 14.Autophagy In Cancer Chemoprevention: Identification of Novel Autophagy Modulators with Anticancer Potential Yuanzhi Lao and Naihan Xu 15. Protocol for a Steady-State FRET Assay in Cancer Chemoprevention Marjolein C. A. Schaap, Andreia M. R. Guimares, Andrew F. Wilderspin, and Geoffrey Wells 16. 3D Tumour Models and Time -Lapse Analysis by Multi-Dimensional Microscopy Dimitri Scholz and Nobue Itasaki 17. Antibody Array as a Tool for Screening of Natural Agents in Cancer Chemoprevention Claudio Pulito, Andrea Sacconi , Anna Maidecchi, Etleva Korita, and Sabrina Strano 18. South African Herbal Extracts as Potential Chemopreventive Agents: Screening for Anti-Cancer Splicing Activity Zodwa Dlamini, Zukile Mbita, and David Bates
