Description
Cancer Cell Signaling: Methods and Protocols, Second Edition brings together the most recent advances in cancer cell signaling knowledge along with recent progress in the development of novel strategies and experimental cell models to study human cancer. Opening with a section on targeting cancer cell metabolism as an anticancer strategy, this detailed volume continues with parts describing protocols to study epigenetic control of cancer, protocols to study cancer metastasis, as well as two new techniques for cancer study. Written for the highly successful Methods in Molecular Biology series, chapters include introductions for their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Authoritative and timely, Cancer Cell Signaling: Methods and Protocols, Second Edition is an ideal guide to the techniques that will aid researchers in exploiting our current knowledge in order to make a profound impact in cancer prevention and treatment. Part I: Targeting Cancer Cell Metabolism as Anticancer Therapy 1. Use of Biguanides to Improve Response to Chemotherapy Vlad C. Sandulache, Liangpeng Yang, and Heath D. Skinner 2. Evaluating Response to Metformin/Cisplatin Combination in Cancer Cells Via Metabolic Measurement and Clonogenic Survival Sang Hyeok Woo, Vlad C. Sandulache, Liangpeng Yang, and Heath D. Skinner 3. Quantifying the Autophagy-Triggering Effects of Drugs in Cell Spheroids with Live Fluorescence Microscopy Nariman Ansari, Stefanie Mller, Katharina Htte, Stefanie Rakel, Patrick Antonietti, Donat Kgel, Ernst H.K. Stelzer, and Francesco Pampaloni Part II: Epigenetic Control of Cancer 4. Interdependency Between Genetic and Epigenetic Regulatory Defects in Cancer Flix Recillas-Targa 5. Experimental Strategies to Manipulate the Cellular Levels of the Multifunctional Factor CTCF Edgar Gonzlez-Buenda, Rosario Prez-Molina, Erandi Ayala-Ortega, Georgina Guerrero, and Flix Recillas-Targa 6. Use of Serum-Circulating miRNA Profiling for the Identification of Breast Cancer Biomarkers Fermn Mar-Aguilar, Cristina Rodrguez-Padilla, and Diana Resndez-Prez 7. Prostate Cancer Detection Using a Non-Invasive Method for Quantifying miRNAs Mauricio Rodrguez-Dorantes, A. Ivan Salido-Guadarrama, and Pilar Garca-Tobilla 8. DNA Methylation Analysis of Steroid Hormone Receptor Genes Ignacio Camacho-Arroyo, Valeria Hansberg-Pastor, and Mauricio Rodrguez-Dorantes 9. Control of Oncogenic miRNA Function by Light-Activated miRNA Antagomirs Colleen M. Connelly and Alexander Deiters 10. Methods for the Study of Long Non-Coding RNA in Cancer Cell Signaling Yi Feng, Xiaowen Hu, Youyou Zhang, Dongmei Zhang, Chunsheng Li, and Lin Zhang Part III: Metastasis Promotion 11. Microvesicles as Mediators of Intercellular Communication in Cancer Marc A. Antonyak and Richard A. Cerione 12. In Vivo Rat Model to Study Horizontal Tumor Progression Catalina Trejo-Becerril, Enrique Prez-Crdenas, and Alfonso Dueas-Gonzlez 13. An In Vivo Model to Study the Effects of Tumoral Soluble Factors on the Vascular Permeability in Mice Cesar Alejandro Guzmn-Prez, Alfredo Ibarra-Snchez, Jos Luis Ventura-Gallegos, Claudia Gonzlez-Espinosa, Jonathan Garca-Romn, and Alejandro Zentella-Dehesa 14. Use of the Tumor Repressor DEDD as a Prognostic Marker of Cancer Metastasis Qi Lv, Fang Hua, and Zhuo-Wei Hu 15. Zebrafish-Based Systems Pharmacology of Cancer Metastasis Yasuhito Shimada, Yuhei Nishimura, and Toshio Tanaka Part IV: New Technologies in the Study of Cancer 16. A Flow Cytometry-Based Assay for the Evaluation of Antibody-Dependent Cell-Mediated Cytotoxicity (ADCC) in Cancer Cells Nohem Salinas-Jazmin, Emiliano Hisaki-Itaya, and Marco A. Velasco-Velzquez 17. Gene Disruption Using Zinc Finger Nuclease Technology Sara Granja, Ibtissam Marchiq, Fatima Baltazar, and Jacques Pouyssgur
