Description
This volume explores the evolution of bacterial cancer therapy and describes the modern techniques used in therapy today. The chapters in this book cover a broad range of topics such as the development of tumor-targeting Salmonella typhimurium A1-R, a microfluidic device for precise quantification of the interactions between tumor-targeting bacteria and tumor tissue, non-invasive in vivo imaging of bacteria-mediated cancer therapy using bio-luminescent bacteria, methods to achieve remote-control of therapeutic gene expression in tumor-targeting bacteria, and cell-cycle decoy of cancer cells resistant to cytotoxic drugs to drug sensitivity by S. typhimurium A1-R. This book concludes with a chapter on the future potential of bacterial therapy of cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and informative, Bacterial Therapy of Cancer: Methods and Protocols is a valuable resource for anyone who is interested in cancer and bacterial therapy. Robert M. Hoffman, Ph.D. Professor of Surgery, University of California, San Diego President, AntiCancer, Inc. 7917 Ostrow Street San Diego, CA 92111 USA TEL: 1-858-654-2555 FAX: 1-858-268-4175 Email: all@anticancer.com 1. Tumor Targeting Salmonella Typhimurium A1-R: An Overview Robert M. Hoffman 2. Enhancement of Tumor-Targeted Delivery of Bacteria with Nitroglycerin Involving Augmentation of the ERP Effect Jun Fang, Lio Long, and Hiroshi Maeda 3. Oral Delivery of Tumor-Targeting Salmonella to Treat Cancer in Mice Dongping Wei and Lijun Jia 4. Microfluidic Device to Quantify the Behavior of Therapeutic Bacteria in Three-Dimensional Tumor Tissue Emily L. Brackett, Charles A. Swofford, and Neil S. Forbes 5. Tumor-Targeting Therapy Using Gene-Engineered Anaerobic-Nonpathogenic Bifidobacterium Longum Shun’ichiro Taniguchi, Yuko Shimatani, and Minoru Fujimori 6. Noninvasive In Vivo Imaging to Follow Bacteria Engaged in Cancer Therapy Sara Lerchner and Siegfried Wiess 7. In Vivo Bioluminescence Imaging of Intra-Tumoral Bacteria Michelle Cronin, Ali R. Akin, Kevin P. Francis, and Mark Tangney 8. Employment of Salmonella in Cancer Gene Therapy Che-Hsin Lee 9. Development of a Targeted Gene Delivery System Using Escherichia Coli Chung-Jen Chiang, Chih-Hsiang Chang, Yun-Peng Chao, and Ming-Ching Kao 10. Isolation and Analysis of Suppressor Mutations in Tumor-Targeted msbB Salmonella K. Brooks Low, Sean R. Murray, John Pawelek, and David Bermudes 11. Determination of Plasmid Segregational Stability in a Growing Bacterial Population M. Gabriela Kramer 12. Visualization of Anti-Cancer Salmonella Typhimurium Engineered for Remote-Control of Therapeutic Proteins Vu H. Hguyen and Jung-Joon Min 13. Methods for Tumor Targeting with Salmonella Typhimurium A1-R Robert M. Hoffman 14. Salmonella Typhimurium A1-R and Cell-Cycle Decoy Therapy of Cancer Robert M. Hoffman 15. Future of Bacterial Therapy of Cancer Robert M. Hoffman
