Description
Herpes Simplex Virus: Methods and Protocols provides a wide collection of protocols employed in various levels of herpes virus research, including basic protocols on growing viruses in cell culture and cloning, manipulating and preparing viral DNA. Other chapters describe approaches to design and apply HSV-1 vectors for vaccination, cancer and gene therapy or to study specific aspects of HSV-1 biology such as latency, intracellular transport and protein-protein interaction. Procedures for structural analyses, microscopy, proteomics and testing of antivirals are included as well. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, Herpes Simplex Virus: Methods and Protocols will aid new researchers in the field of herpes virology as well as those experienced investigators wishing to embark on new techniques. 1. HSV-1 Biology and Life Cycle Roger D. Everett 2. Herpes Simplex Virus Growth, Preparation, and Assay Peggy Marconi and Roberto Manservigi 3. Isolation of Herpes Simplex Virus Nucleocapsid DNA Moriah Szpara 4. Construction and Characterization of Bacterial Artificial Chromosomes (BACs) Containing Herpes Simplex Virus Full-Length Genomes Claus-Henning Nagel, Anja Pohlmann, and Beate Sodeik 5. Engineering HSV-1 Vectors for Gene Therapy William F. Goins, Shaohua Huang, Justus B. Cohen, and Joseph C. Glorioso 6. Herpes Simplex Virus Type 1 (HSV-1)-Derived Amplicon Vectors Matias E. Melendez, Cornel Fraefel, and Alberto L. Epstein 7. HSV-1 Amplicon Vectors as Genetic Vaccines Andrea S. Laimbacher and Cornel Fraefel 8. Modification of HSV-1 to an Oncolytic Virus Hiroshi Nakashima and E. Antonio Chiocca 9. Herpes Simplex Virus Mutant Generation and Dual Detection Methods for Gaining Insight into Latent/Lytic Cycles In Vivo Nancy M. Sawtell and Richard L. Thompson 10. Phenotypic and Genotypic Testing of HSV-1 Resistance to Antivirals Andreas Sauerbrei and Kathrin Bohn 11. Using Homogeneous Primary Neuron Cultures to Study Fundamental Aspects of HSV-1 Latency and Reactivation Ju Youn Kim, Lora A. Shiflett, Jessica A. Linderman, Ian Mohr, and Angus C. Wilson 12. Characterization of Extracellular HSV-1 Virions by Proteomics Roger Lipp 13. A Precipitation-Based Assay to Analyze Interactions of Viral Particles with Cytosolic Host Factors Kerstin Radtke, Fenja Anderson, and Beate Sodeik 14. Affinity Purification Combined with Mass Spectrometry to Identify Herpes Simplex Virus Protein-Protein Interactions David G. Meckes, Jr. 15. Preparation of Herpes Simplex Virus Infected Primary Neurons for Transmission Electron Microscopy Monica Miranda-Saksena, Ross Boadle, and Anthony L. Cunningham 16. Multifluorescence Live Analysis of Herpes Simplex Virus Type-1 Replication Michael Seyffert, Anna Paula de Oliveira, Cornel Fraefel, and Rebecca Vogel 17. Expression, Purification, and Crystallization of HSV-1 Glycoproteins for Structure Determination Samuel D. Stampfer and Ekaterina E. Heldwein 18. Cryo-EM Techniques to Resolve the Structure of HSV-1 Capsid-Associated Components Ryan H. Rochat, Corey W. Hecksel and Wah Chiu 19. In Vivo HSV-1 DNA Transport Studies Using Murine Retinal Ganglion Cells Jolene M. Draper, Graham S. Stephenson and Jennifer H. LaVail 20. HSV-1 Protein Expression Using Recombinant Baculoviruses Lorry M. Grady, Ping Bai and Sandra K. Weller 21. The Murine Intravaginal HSV-2 Challenge Model for Investigation of DNA Vaccines Joshua O. Marshak, Lichun Dong, and David M. Koelle
